神经生长因子
血管生成
骨整合
血管内皮生长因子
体内
医学
新生血管
原肌球蛋白受体激酶A
绒毛尿囊膜
生长因子
细胞生物学
植入
内分泌学
内科学
生物
外科
受体
血管内皮生长因子受体
生物技术
作者
Mengkai Guang,Yang Yao,L. Zhang,Bo Huang,Li Ma,Lin Xiang,Jian Jin,Pengjian Gong
标识
DOI:10.1016/j.ijom.2015.06.016
摘要
Angiogenesis is critical for peri-implant bone regeneration and osseointegration. Endothelial cells (ECs) play an important role in angiogenesis during the early stage of bone formation. Nerve growth factor (NGF) is also reported to function as an angiogenic growth factor. The effects of NGF on ECs seeded on titanium surfaces are unclear. This study was done to investigate the influence of NGF on peri-implant angiogenesis in vitro and in vivo. We used two different titanium surfaces. ECs seeded on these surfaces were treated with indicated concentrations of NGF or vascular endothelial growth factor (VEGF). Proliferation, differentiation, morphological features, and amounts attached were assessed. Chicken embryo chorioallantoic membrane (CAM) was adopted to evaluate the effect of NGF in vivo. The results showed that NGF could promote EC proliferation on both titanium surfaces (F1d = 2.083, P = 0.156; F3d = 30.857, P = 0.0002; F5d = 4.440, P = 0.041; F7d = 11.065, P = 0.001). NGF and the SLA surface upregulated mRNA of NGF, TrkA, and p75 expression (FNGF = 11.941, P = 0.003; FTrkA = 28.514, P = 0.004; Fp75 = 7.725, P = 0.01). In vivo, the supernatants of the NGF-treated group could promote neovascularization in CAM (F = 17.662, P = 0.009). This study demonstrated that NGF could enhance EC proliferation, gene expression on different titanium surfaces, and neovascularization in CAM. This provides novel information in relation to the promotion of early dental implant osseointegration.
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