细胞因子
卵清蛋白
B细胞
生物
T细胞
免疫学
内科学
内分泌学
分子生物学
化学
抗原
免疫系统
医学
抗体
作者
Masato Tsuda,Haruka Arakawa,Narumi Ishii,Chihiro Ubukata,Mana Michimori,Masanari Noda,Kyôko Takahashi,Shuichi Kaminogawa,Akira Hosono
出处
期刊:International Archives of Allergy and Immunology
[S. Karger AG]
日期:2017-01-01
卷期号:174 (3-4): 121-132
被引量:24
摘要
<b><i>Background:</i></b> Fructo-oligosaccharides (FOS) are prebiotic agents with immunomodulatory effects involving improvement of the intestinal microbiota and metabolome. In this study, we investigated the cellular mechanisms through which FOS modulate intestinal antigen-specific CD4<sup>+</sup> T cell responses in food allergy, using OVA23-3 mice. <b><i>Methods:</i></b> OVA23-3 mice were fed an experimental diet containing either ovalbumin (OVA) or OVA and FOS for 1 week. Body weight and mucosal mast cell protease 1 in the serum were measured as the indicator of intestinal inflammation. Single-cell suspensions were prepared from intestinal and systemic lymphoid tissues for cellular analysis. Cytokine production was measured by ELISA. Activation markers and intracellular cytokines in CD4<sup>+</sup> T cells were analyzed by flow cytometry. Activated CD4<sup>+</sup> T cells were purified to examine cytokine production. <b><i>Results:</i></b> Dietary intake of FOS provided moderate protection from the intestinal inflammation induced by the OVA-containing diet. FOS significantly reduced food allergy-induced Th2 cytokine responses in intestinal tissues but not in systemic tissues. FOS decreased OVA diet-induced IFN-γ<sup>+</sup>IL-4<sup>+</sup> double-positive CD4<sup>+</sup> T cells and early-activated CD45RB<sup>high</sup>CD69<sup>+</sup>CD4<sup>+</sup> T cells in the mesenteric lymph nodes. Furthermore, we confirmed that these CD45RB<sup>high</sup>CD69<sup>+</sup>CD4<sup>+</sup> T cells are able to produce high levels of IFN-γ and moderate level of IL-4, IL-10, and IL-13. <b><i>Conclusions:</i></b> Dietary intake of FOS during the development of food allergy attenuates the induction of intestinal Th2 cytokine responses by regulating early activation of naïve CD4<sup>+</sup> T cells, which produce both Th1 and Th2 cytokines. Our results suggest FOS might be a potential food agent for the prevention of food allergy by modulating oral sensitization to food antigens.
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