化学
闪光光解
放射分析
鸟嘌呤
光化学
激进的
羟基自由基
自由基离子
电子转移
光敏剂
光解
键裂
动力学
反应速率常数
离子
有机化学
催化作用
生物化学
核苷酸
基因
物理
量子力学
作者
Lingli Cheng,Yujia Wang,Dahong Huang,Side Yao,Guangwei Ding,Shilong Wang,Zheng Jiao
标识
DOI:10.1016/j.saa.2014.01.085
摘要
In this paper, the photochemical and photobiological characters of the active radicals of berberine (BBR) was investigated for finding an efficient and safe photosensitizer with highly active transient products using in Photodynamic therapy (PDT) study. The active species of BBR was generated and identified by using pulse radiolysis method. In neutral aqueous solution, BBR react with hydrated electron and hydroxyl radical, forming the radical anion and neutral radical of BBR, and the related reaction rates were determined as 3.5×10(10) and 6.7×10(9) M(-1) s(-1), respectively. Further, the capability of BBR to photosensitize DNA cleavage was testified by laser flash photolysis (LFP) method, the results demonstrated that BBR neutral radical could react with guanine mononucleotide (K=1.9×10(9) M(-1) s(-1)) via electron transfer to give the guanine neutral radical. Additionally BBR selective cleavage single and double strand DNA at guanine moiety was observed. Finally, combining with the thermodynamic calculation, the possible photodamage mechanism of dGMP and DNA induced by BBR was clarified.
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