化学
硫黄素
电化学
氧化还原
生物物理学
肽
降级(电信)
低聚物
荧光
体外
动态光散射
循环伏安法
电极
材料科学
纳米技术
生物化学
阿尔茨海默病
纳米颗粒
无机化学
有机化学
物理化学
电信
生物
医学
病理
量子力学
疾病
计算机科学
物理
作者
Baole Zhang,Tao Zhu,Lei Liu,Yanhui Liang
标识
DOI:10.1016/j.jcis.2022.09.009
摘要
The clearance of overloaded amyloid β (Aβ) oligomers is thought to be an attractive and potential strategy for the therapy of Alzheimer's disease (AD). A variety of strategies have already been utilized to study Aβ degradation in vitro. Here, the electrochemical detection based on direct electrooxidation of specific Tyr residues within Aβ peptide has been developed as a simple and robust approach for monitoring the oligomers' degradation. C60 was employed for photodegrading Aβ oligomers due to the generated ROS under light irradiation. The oxidation current of Tyr residues by square wave voltammetry (SWV) increased upon the Aβ degradation, confirming that the structure variation of Aβ peptide indeed influenced the exposure of those redox species to the electrode surface and final signal output. Chronoamperometric assay also found the electrooxidation of Tyr undergone an irreversible process. Additionally, the direct electrochemistry was capable of detecting the aggregation with rapid test and better sensitivity in compared with dynamic light scattering (DLS), atomic force microscopy (AFM) and thioflavin T (ThT) based fluorescence assay. Thus, this work indicated the potential application of direct electrochemistry in the in vitro measurement of Aβ degradation and clearance, providing new insights and a complementary means into the AD theranostics.
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