肝损伤
化学
对乙酰氨基酚
谷胱甘肽
药品
半胱氨酸
荧光
体内
内生
药理学
雷公藤甲素
肝功能
戒毒(替代医学)
生物化学
酶
内科学
病理
医学
细胞凋亡
生物
物理
替代医学
生物技术
量子力学
作者
Feiyan Liu,Yalin Li,Jiangmin Zhu,Li Yin,Di Zhu,Jian‐Guang Luo,Ling‐Yi Kong
标识
DOI:10.1016/j.bioorg.2023.106899
摘要
Drug-induced liver injury (DILI), induced by overdose or chronic administration of drugs, has become the leading cause of acute liver failure. Therefore, an accurate diagnostic method for DILI is critical to improve treatment efficiency. The production of γ-glutamyltranspeptidase (GGT) is closely related to the progression of drug-induced hepatotoxicity. KL-Glu exhibits a prominent GGT-activated NIR fluorescence (734 nm) with a large Stokes shift (137 nm) and good sensitivity/selectivity, making it favorable for real-time detection of endogenous GGT activity. Using this probe, we evaluated the GGT up-regulation under the acetaminophen-induced liver injury model. Moreover, KL-Glu was successfully used to assess liver injury induced by the natural active ingredient triptolide and the effective amelioration upon treatment with N-acetyl cysteine (NAC) or Glutathione (GSH) in cells and in vivo by fluorescent trapping the fluctuation of GGT for the first time. Therefore, the fluorescent probe KL-Glu can be used as a potential tool to explore the function of GGT in the progression of DILI and for the early diagnosis and prognostic evaluation of DILI.
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