Encapsulated laccase in bimetallic Cu/Zn ZIFs as stable and reusable biocatalyst for decolorization of dye wastewater

Laccase have received extensive attention in pollutant degradation, but its practical viability is largely affected by the poor stability, easy inactivation and difficulty in recycling for the present. Enzyme immobilization offers enhanced enzyme stability and constructs a synergistic system for the efficient adsorption and degradation of pollutants. In this study, bimetallic Cu/Zn ZIFs were synthesized by co-precipitation method as the protective carrier for laccase. Lac@Cu-ZIF-90 exhibited a good protective effect on laccase and showed a high operational stability in various interfering environments. Free laccase was completely inactivated at pH 7.0 but Lac@Cu-ZIF-90 could maintain 50.0 % activity. Benefiting from the encapsulation of laccase and porous structure of Cu-ZIF-90, the Lac@Cu-ZIF-90 exhibited decolorization efficiency for dye wastewater. More importantly, the Lac@Cu-ZIF-90 could be recovered from the dye solution and re-used to adsorb and degrade the synthetic dye for multiple times, its removal rate for reactive deep green was only decreased about 10.8 % after five cycles. This work reveals that the Cu-ZIF-90 provides a favorable environment for laccase and as a protective layer to relieve the conformation change, which provides an efficient strategy to decolorize dye wastewater. Therefore, Cu-ZIF-90 promises applications as enzymes encapsulation has great potential in water remediation.