抗原
免疫学
免疫系统
CD8型
医学
表位
外周血淋巴细胞
细胞毒性T细胞
肽疫苗
生物
体外
生物化学
作者
Qiyuan Chen,Heather Jackson,Mark Shackleton,Phillip Parente,Wendie Hopkins,Sue Sturrock,Duncan MacGregor,Eugene Maraskovsky,Tsin Yee Tai,Nektaria Dimopoulos,Kelly‐Anne Masterman,Tina Luke,Ian D. Davis,Weisan Chen,Jonathan Cebon
出处
期刊:PubMed
日期:2005-03-09
卷期号:5: 5-5
被引量:5
摘要
Immune responses to cancer vaccines are commonly tested by measuring cutaneous reactions to intradermal (i.d.) antigen. When well-characterized peptide epitopes are injected i.d., infiltrates of CD4+ and CD8+ T lymphocytes are frequently seen. In this study, we have further characterized T cells derived from vaccine-infiltrating lymphocyte (VIL) responses. We found that the infiltrates capable of producing IFN-gamma and cytolytic activity could recognize vaccine peptide, as well as antigen-positive melanoma cells. We studied antigen-specific T cell responses from VILs and peripheral blood in 10 patients who participated in a clinical trial. All patients received systemic Flt3 ligand (20 microg/kg/d) and i.d. peptides: Three NY-ESO-1 peptides, SLLMWITQCFL (157-167), SLLMWITQC (157-165), QLSLLMWIT (155-163); tyrosinase internal peptide YMDGTMSQV (368-376); Melan-A/MART-1 analogue peptide ELAGIGILTV (26-35, E27L substitution); and influenza matrix peptide GILGFVFTL (58-66). In 54 paired VIL and peripheral blood analyses, a good correlation was found between responses in skin and in blood. These cells could be rapidly expanded in a short-term assay and thus appear to be memory T cells. The demonstrated presence of antigen-specific T cells at vaccination sites validates this method of assessing the immune response to i.d. vaccines.
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