RNA沉默
RNA干扰
核糖核酸
生物
细胞生物学
基因沉默
基因
遗传学
作者
Andreas Voloudakis,Maria C. Holeva,L. Peter Sarin,Dennis H. Bamford,Marisol Vargas,Minna M. Poranen,Francisco Tenllado
出处
期刊:Methods in molecular biology
日期:2014-09-16
卷期号:: 255-274
被引量:51
标识
DOI:10.1007/978-1-4939-1743-3_19
摘要
Double-stranded RNA (dsRNA) is an inducer molecule of the RNA silencing (RNA interference, RNAi) pathway that is present in all higher eukaryotes and controls gene expression at the posttranscriptional level. This mechanism allows the cell to recognize aberrant genetic material in a highly sequence specific manner. This ultimately leads to degradation of the homologous target sequence, rendering the plant cell resistant to subcellular pathogens. Consequently, dsRNA-mediated resistance has been exploited in transgenic plants to convey resistance against viruses. In addition, it has been shown that enzymatically synthesized specific dsRNA molecules can be applied directly onto plant tissue to induce resistance against the cognate virus. This strongly implies that dsRNA molecules are applicable as efficacious agents in crop protection, which will fuel the demand for cost-effective dsRNA production methods. In this chapter, the different methods for dsRNA production-both in vitro and in vivo-are described in detail.
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