膜
微型多孔材料
材料科学
静电纺丝
多孔性
化学工程
纳米纤维
检出限
化学
色谱法
纳米技术
聚合物
复合材料
生物化学
工程类
作者
Cunyi Zhao,Bofeng Pan,Minyuan Wang,Yang Si,Ameer Y. Taha,Gang-yu Liu,Tingrui Pan,Gang Sun
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2022-04-15
卷期号:7 (5): 1458-1466
被引量:13
标识
DOI:10.1021/acssensors.2c00208
摘要
An ultrasensitive and portable colorimetric enzyme-linked immunosorbent assay (ELISA) sensor for antibiotics was fabricated by immobilizing antibodies inside the largely porous and highly hydrophilic nanofibrous membranes. Different from regular electrospun nanofibrous membranes where antibodies may frequently be blocked by the heterogeneous porous structure and sterically crowded loaded on the surface, the controlled microporous structure and increased hydrophilicity of nanofibrous membranes could improve the diffusion properties of antibodies, reduce the sterically crowding effect, and dramatically improve the sensitivity of the membrane-based ELISA. The limitation of detection (LOD) for chloramphenicol (CAP) reached 0.005 ng/mL, around 200 times lower than the conventional paper-based ELISA, making quantitative analysis and portable on-site detection achievable via the use of smartphones. The successful design and fabrication of the nanofibrous membrane-based ELISA with novel features overcome the structural drawbacks of regular electrospun nanofibrous membranes and provide new paths to develop highly sensitive on-site detection of hazardous chemical agents.
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