信号转导衔接蛋白
细胞生物学
生物
维甲酸
信号转导
受体
先天免疫系统
生物化学
基因
作者
Peng Zhang,Anna D. Reichardt,Huanhuan Liang,Roghiyh Aliyari,David Cheng,Yaya Wang,Feng Xu,Genhong Cheng,Yingfang Liu
出处
期刊:Science Signaling
[American Association for the Advancement of Science (AAAS)]
日期:2012-11-13
卷期号:5 (250)
被引量:34
标识
DOI:10.1126/scisignal.2003152
摘要
The TRAF [tumor necrosis factor receptor-associated factor] family of cytoplasmic adaptor proteins link cell-surface receptors to intracellular signaling pathways that regulate innate and adaptive immune responses. In response to activation of RIG-I (retinoic acid-inducible gene I), a component of a pattern recognition receptor that detects viruses, TRAF3 binds to the adaptor protein Cardif [caspase activation and recruitment domain (CARD) adaptor-inducing interferon-β (IFN-β)], leading to induction of type I IFNs. We report the crystal structures of the TRAF domain of TRAF5 and that of TRAF3 bound to a peptide from the TRAF-interacting motif of Cardif. By comparing these structures, we identified two residues located near the Cardif binding pocket in TRAF3 (Tyr(440) and Phe(473)) that potentially contributed to Cardif recognition. In vitro and cellular experiments showed that forms of TRAF5 with mutation of the corresponding residues to those of TRAF3 had TRAF3-like antiviral activity. Our results provide a structural basis for the critical role of TRAF3 in activating RIG-I-mediated IFN production.
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