适体
细胞毒性
化学
介孔二氧化硅
药物输送
MUC1号
共轭体系
纳米颗粒
Zeta电位
粒径
生物物理学
纳米技术
材料科学
体外
介孔材料
生物化学
有机化学
粘蛋白
分子生物学
催化作用
聚合物
物理化学
生物
作者
Mohammad Yahya Hanafi‐Bojd,Seyedeh Alia Moosavian Kalat,Seyed Mohammad Taghdisi,Legha Ansari,Khalil Abnous,Bizhan Malaekeh‐Nikouei
标识
DOI:10.1080/03639045.2017.1371734
摘要
In the present study, we developed aptamer (Apt) conjugated mesoporous silica nanoparticles (MSNs) for specific delivery of epirubicin (EPI) to breast cancer cells. MSNs were synthesized and functionalized with 3-mercaptopropyltrimethoxysilane (3-MPTMS), followed by MUC1 aptamer conjugation through disulfide bonds. The nanoparticles were analyzed by transmission electron microscopy (TEM), particle size analyzer, zeta potential, elemental analysis (CHNS), aptamer conjugation efficiency, drug loading efficiency, and drug release profile. Cell uptake and in vitro cytotoxicity of different formulations were performed. The results of MSNs characterization confirmed spherical nanoparticles with thiol functional groups. Particle size of obtained nanoparticles was 163 nm in deionized water. After conjugation of MUC1 aptamer and EPI loading (MSN-MUC1-EPI), particle size increased to 258 nm. The aptamer conjugation to MSNs with disulfide bonds were confirmed using gel retardation assay. Cellular uptake studies revealed better cell uptake of MSN-MUC1-EPI compared to MSN-EPI. Moreover, cytotoxicity study results in MCF7 cell lines showed improved cytotoxicity of MSN-MUC1-EPI in comparison with MSN-EPI or EPI at the same concentration of drug. These results exhibited that MSN-MUC1-EPI has the potential for targeted drug delivery into MUC1 positive breast cancer cells to improve drug efficacy and alleviate side effects.
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