细胞凋亡
细胞周期
细胞周期检查点
DNA断裂
生物
DNA损伤
细胞生物学
程序性细胞死亡
化学
分子生物学
癌症研究
生物化学
DNA
作者
Yujie Fu,Onat Kadioglu,Benjamin Wiench,Zuo‐Fu Wei,Chang Gao,Meng Luo,Cheng‐Bo Gu,Yuangang Zu,Thomas Efferth
出处
期刊:Phytomedicine
[Elsevier]
日期:2015-04-01
卷期号:22 (4): 462-468
被引量:35
标识
DOI:10.1016/j.phymed.2015.02.005
摘要
Background: The low abundant cajanin stilbene acid (CSA) from Pigeon Pea (Cajanus cajan) has been shown to kill estrogen receptor α positive cancer cells in vitro and in vivo. Downstream effects such as cell cycle and apoptosis-related mechanisms have not been analyzed yet. Material and methods: We analyzed the activity of CSA by means of flow cytometry (cell cycle distribution, mitochondrial membrane potential, MMP), confocal laser scanning microscopy (MMP), DNA fragmentation assay (apoptosis), Western blotting (Bax and Bcl-2 expression, caspase-3 activation) as well as mRNA microarray hybridization and Ingenuity pathway analysis. Results: CSA induced G2/M arrest and apoptosis in a concentration-dependent manner from 8.88 to 14.79 µM. The MMP broke down, Bax was upregulated, Bcl-2 downregulated and caspase-3 activated. Microarray profiling revealed that CSA affected BRCA-related DNA damage response and cell cycle-regulated chromosomal replication pathways. Conclusion: CSA inhibited breast cancer cells by DNA damage and cell cycle-related signaling pathways leading to cell cycle arrest and apoptosis.
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