适体
费斯特共振能量转移
检出限
大肠杆菌
纳米颗粒
荧光
化学
致病菌
细菌
胶体金
氧化铈
线性范围
组合化学
分析化学(期刊)
色谱法
核化学
材料科学
氧化物
纳米技术
生物化学
生物
有机化学
分子生物学
物理
基因
量子力学
遗传学
作者
Sena Ghayyem,Farnoush Faridbod
出处
期刊:Analytical Methods
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:14 (8): 813-819
被引量:2
摘要
Herein, we present a facile and sensitive fluorescence resonance energy transfer (FRET) aptasensor for the detection of pathogenic bacteria, where antibiotic-functionalized cerium oxide nanoparticles were served as an energy donor and aptamer-modified gold nanoparticles (aptamer-AuNPs) were employed as an energy acceptor. To illustrate the feasibility of this strategy, Escherichia coli (E. coli) was examined. The strategy for the detection of E. coli bacteria as a target molecule is described using the FRET pair of azithromycin-functionalized CeO2 nanoparticles (Azm-CeO2NPs) and aptamer-AuNPs. The spectral overlap between these two nanoparticles and Azm and the aptamer binding on the surface of E. coli specifically provides the condition, which leads to the occurrence of the FRET phenomenon. In this way, a good linear correlation between the fluorescence intensity of Azm-CeO2NPs and E. coli concentration was obtained in the range of 10-1.5 × 105 cfu mL-1. The detection limit of the proposed method at a signal to noise ratio of 3 (3σ) was estimated to be 1.04 cfu mL-1. Further, the proposed method was applied to detect E. coli in real samples within 30 min, which indicates the applicability of the proposed method. This method could be used for other pathogenic bacterium recognition or synchronous detection by employing molecules that are particular to the desired bacteria.
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