纳米载体
丝胶
药物输送
泊洛沙姆
紫杉醇
细胞毒性
材料科学
膜联蛋白
生物物理学
动态光散射
纳米颗粒
细胞凋亡
纳米技术
体外
化学
丝绸
生物化学
癌症
聚合物
生物
共聚物
复合材料
遗传学
作者
Biman B. Mandal,Subhas C. Kundu
出处
期刊:Nanotechnology
[IOP Publishing]
日期:2009-08-11
卷期号:20 (35): 355101-355101
被引量:119
标识
DOI:10.1088/0957-4484/20/35/355101
摘要
In recent times self-assembled micellar nanoparticles have been successfully employed in tissue engineering for targeted drug delivery applications. In this review, silk sericin protein from non-mulberry Antheraea mylitta tropical tasar silk cocoons was blended with pluronic F-127 and F-87 in the presence of solvents to achieve self-assembled micellar nanostructures capable of carrying both hydrophilic (FITC-inulin) and hydrophobic (anticancer drug paclitaxel) drugs. The fabricated nanoparticles were subsequently characterized for their size distribution, drug loading capability, cellular uptake and cytotoxicity. Nanoparticle sizes ranged between 100 and 110 nm in diameter as confirmed by dynamic light scattering. Rapid uptake of these particles into cells was observed in in vitro cellular uptake studies using breast cancer MCF-7 cells. In vitro cytotoxicity assay using paclitaxel-loaded nanoparticles against breast cancer cells showed promising results comparable to free paclitaxel drugs. Drug-encapsulated nanoparticle-induced apoptosis in MCF-7 cells was confirmed by FACS and confocal microscopic studies using Annexin V staining. Up-regulation of pro-apoptotic protein Bax, down-regulation of anti-apoptotic protein Bcl-2 and cleavage of regulatory protein PARP through Western blot analysis suggested further drug-induced apoptosis in cells. This study projects silk sericin protein as an alternative natural biomaterial for fabrication of self-assembled nanoparticles in the presence of poloxamer for successful delivery of both hydrophobic and hydrophilic drugs to target sites.
科研通智能强力驱动
Strongly Powered by AbleSci AI