灰葡萄孢菌
激发子
丁香假单胞菌
葡萄球菌炎
生物
过敏反应
微生物学
病原真菌
分子质量
植物抗病性
糖蛋白
植保素
真菌
系统获得性抵抗
植物对草食的防御
病菌
生物化学
酶
植物
基因
拟南芥
突变体
白藜芦醇
作者
Yi Zhang,Yunhua Zhang,Dewen Qiu,Hongmei Zeng,Lanping Guo,Xiufen Yang
标识
DOI:10.1016/j.bbrc.2015.01.038
摘要
In this study, a necrosis-inducing protein was purified from the culture filtrate of the necrotrophic fungus Botrytis cinerea BC-98 strain. Secreted proteins were collected and fractionated by liquid chromatography. The fraction with the highest necrosis-inducing activity was further purified. A glycoprotein named BcGs1 was identified by 2D electrophoresis and mass spectrometry. The BcGs1 protein consisted of 672 amino acids with a theoretical molecular weight of 70.487 kDa. Functional domain analysis indicated that BcGs1 was a glucan 1,4-alpha-glucosidase, a cell wall-degrading enzyme, with a Glyco_hydro_15 domain and a CBM20_glucoamylase domain. The BcGs1 protein caused necrotic lesions that mimicked a typical hypersensitive response and H2O2 production in tomato and tobacco leaves. BcGs1-treated plants exhibited resistance to B. cinerea, Pseudomonas syringae pv. tomato DC3000 and tobacco mosaic virus in systemic leaves. In addition, BcGs1 triggered elevation of the transcript levels of the defence-related genes PR-1a, TPK1b and Prosystemin. This is the first report of a Botrytis glucan 1,4-alpha-glucosidase triggering host plant immunity as an elicitor. These results lay a foundation for further study of the comprehensive interaction between plants and necrotrophic fungi.
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