十二烷基硫酸钠
聚丙烯酰胺凝胶电泳
色谱法
凝胶电泳
钠
化学
二维凝胶电泳
蛋白质凝胶电泳
电泳
甘油
聚丙烯酰胺
生物化学
高分子化学
有机化学
酶
蛋白质组学
基因
作者
Xiaoqiang Wu,Hisashi Koiwa
标识
DOI:10.1016/j.ab.2011.10.004
摘要
A modified Laemmli sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protocol is described. The new method saves 30 min for gel casting without loss of the resolution power of Laemmli gel. In this method, both the upper and lower gels can be cast at the same time because the lower gel contains 10% glycerol, which generates higher density in the lower gel than in the upper gel.
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