化学
适体
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
胶体金
热点(地质)
拉曼光谱
2019年冠状病毒病(COVID-19)
纳米颗粒
纳米技术
分子生物学
材料科学
生物
物理
医学
疾病
病理
传染病(医学专业)
光学
地球物理学
作者
Guang-Yao Huang,Hongxin Zhao,Pan Li,Juanjuan Liu,Siyu Chen,Meihong Ge,Qiguang Miao,Guoliang Zhou,Yongtao Wang,Shaofei Li,Yuanhui Cheng,Qiang Huang,Junfeng Wang,Hongzhi Wang,Liangbao Yang
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2021-11-03
卷期号:93 (48): 16086-16095
被引量:22
标识
DOI:10.1021/acs.analchem.1c03807
摘要
It is highly challenging to construct the best SERS hotspots for the detection of proteins by surface-enhanced Raman spectroscopy (SERS). Using its own characteristics to construct hotspots can achieve the effect of sensitivity and specificity. In this study, we built a fishing mode device to detect the receptor-binding domain (RBD) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at low concentrations in different detection environments and obtained a sensitive SERS signal response. Based on the spatial resolution of proteins and their protein-specific recognition functions, SERS hotspots were constructed using aptamers and small molecules that can specifically bind to RBD and cooperate with Au nanoparticles (NPs) to detect RBD in the environment using SERS signals of beacon molecules. Therefore, two kinds of AuNPs modified with aptamers and small molecules were used in the fishing mode device, which can specifically recognize and bind RBD to form a stable hotspot to achieve high sensitivity and specificity for RBD detection. The fishing mode device can detect the presence of RBD at concentrations as low as 0.625 ng/mL and can produce a good SERS signal response within 15 min. Meanwhile, we can detect an RBD of 0.625 ng/mL in the mixed solution with various proteins, and the concentration of RBD in the complex environment of urine and blood can be as low as 1.25 ng/mL. This provides a research basis for SERS in practical applications for protein detection work.
科研通智能强力驱动
Strongly Powered by AbleSci AI