CD47型
基因敲除
细胞凋亡
癌症研究
细胞培养
吞噬作用
癌细胞
细胞生长
下调和上调
癌症
化学
生物
医学
细胞
免疫学
内科学
遗传学
基因
生物化学
作者
Xiaojing Ye,Jianguang Yang,Ya-Qin Tan,Xiaojie Chen,Gang Zhou
出处
期刊:Anti-cancer Agents in Medicinal Chemistry
[Bentham Science]
日期:2021-04-01
卷期号:21 (6): 766-774
被引量:2
标识
DOI:10.2174/1871520620999200730162915
摘要
Background: Our previous work demonstrated upregulated CD47 in oral squamous cell carcinoma (OSCC). Objective: In the present study,we aimed to investigate the effects of CD47 on tumor cell development and phagocytosis in OSCC and elucidate the underlying mechanisms. Methods: The proliferation, apoptosis, migration, and invasion of oral cancer cells were analyzed after knocking down the expression of CD47. The effects of CD47 on tumor development were also evaluated using a murine model of OSCC. The involvement of CD47 in the phagocytosis of oral cancer cells was identified. Results: Cell proliferation was suppressed by knocking down the expression of CD47 in human OSCC cell line Cal-27 cells but there was no change in theapoptosis rate. Moreover, impaired expression of CD47 inhibited the migration and invasion of Cal-27 cells. Furthermore, we found that nude mice injected with CD47 knocked-down Cal-27 cells displayed decreased tumor volumes at week 9 compared to xenograft transplantations of blank Cal-27 cells. In addition, in vitrophagocytosis of Cal-27 cells by macrophages was significantly enhanced after the knockdown of CD47, which positively correlated with compromised STAT3/JAK2 signaling. Conclusion: In summary, the knockdown of CD47 down regulated the development of OSCC and increased the phagocytosis of Cal-27 cells, indicating that CD47 might be a promising therapeutic target.
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