大黄素
软骨
体内
骨关节炎
化学
药理学
体外
塞来昔布
基质金属蛋白酶
医学
病理
生物化学
解剖
生物
生物技术
替代医学
作者
Hailong Hu,Xiaopeng Song,Yue Li,Tianwen Ma,Hui Bai,Mingchao Zhao,Xinyu Wang,Lin Liu,Gao Li
出处
期刊:Life Sciences
[Elsevier]
日期:2021-03-01
卷期号:269: 119001-119001
被引量:17
标识
DOI:10.1016/j.lfs.2020.119001
摘要
Osteoarthritis (OA) is a common joint disease and the main cause of disability. We sought to determine the effective concentration of emodin on chondrocytes and to identify the dosage of emodin that induces a comparable therapeutic effect with the COX-2 inhibitor drug, celecoxib that is currently used to treat OA. In vitro experiments induced inflammation of chondrocytes by IL-1β, and an osteoarthritis model was established in vivo by cutting rat anterior cruciate ligament. Western Blot, Real-time PCR, HE staining, Safranin O-green staining and immunohistochemistry were performed to detect MMP-3, MMP-13, ADAMTS-4, iNOS and COL2A1 on the chondrocytes or the tibial plateau. The cytokine activity and content in serum of six groups of rats were measured by kit. It was found that the surface layer of the cartilage was thicker and smoother after the administration of emodin. Tissue expression of MMP-3, MMP-13, ADAMTS-4 and iNOS were significantly (p < 0.05) decreased in chondrocytes and cartilage treated with different doses of emodin, and the content of COL2A1 was reversed. Emodin also significantly decreased the blood levels of COX-2 and PGE2. The effective emodin in vitro was 5 μmol/L, whereas emodin at 80 mg/kg was equivalent to celecoxib in vivo. Emodin reduces the expression of cartilage matrix degradation biomarkers, thereby reducing the degradation of cartilage matrix and protecting the knee joint cartilage. Emodin at 5 μmol/L shows the best concentration to treat chondrocytes, and the protective effect of emodin at 80 mg/kg is comparable to that of celecoxib.
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