间充质干细胞
运行x2
发病机制
下调和上调
生物
癌症研究
细胞分化
细胞生物学
长非编码RNA
转录因子
免疫学
基因
遗传学
作者
Qianyu Zhuang,Buqing Ye,Shangyi Hui,Ying Du,Robert Chunhua Zhao,Jing Li,Zhihong Wu,Na Li,Yanbin Zhang,Hongling Li,Shengru Wang,Yang Yang,Shugang Li,Hong Zhao,Zusen Fan,Guixing Qiu,Jianguo Zhang
标识
DOI:10.1038/s41418-018-0240-2
摘要
Adolescent idiopathic scoliosis (AIS) is a complex, three dimensional deformity of the spine that commonly occurs in pubescent girls. Abnormal osteogenic differentiation of mesenchymal stem cells (MSCs) is implicated in the pathogenesis of AIS. However, the biological roles of long noncoding RNAs (lncRNAs) in the regulation of osteogenic differentiation of MSCs are unknown. Through microarray analyses of bone marrow (BM) MSCs from healthy donors and AIS patients, we identified 1483 differentially expressed lncRNAs in AIS BM-MSCs. We defined a novel lncAIS (gene symbol: ENST00000453347) is dramatically downregulated in AIS BM-MSCs. In normal BM-MSCs, lncAIS interacts with NF90 to promote HOXD8 mRNA stability that enhances RUNX2 transcription in BM-MSCs, leading to osteogenic differentiation of normal BM-MSCs. By contrast, lncAIS downregualtion in AIS BM-MSCs cannot recruit NF90 and abrogates HOXD8 mRNA stability, which impedes RUNX2 transcription for osteogenic differentiation. Thereby lncAIS downregualtion in BM-MSCs suppresses osteogenic differentiation that is implicated in the pathogenesis of AIS.
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