Palladium-Platinum Bimetallic Modified MXene Nanozyme for Highly Sensitive Detection of Active Substances with Acetylcholinesterase Inhibitory Effect

Alzheimer's disease (AD) is a chronic neurodegenerative disease that threatens to the health of global elderly population. Acetylcholinesterase (AChE) inhibitors are an effective therapeutic agent for AD, and screening of these substances is important for AD treatment. In this work, a Pd-Pt MXene nanoenzyme was successfully synthesized by using the in-situ reduction technique. A colorimetric method for sensitive AChE inhibitor detection was designed based on enzymatic cascade reaction between Pd-Pt MXene and AChE. Briefly, The Pd-Pt MXene material exhibited excellent peroxidase (POD)-like activity due to its bimetallic composition, effectively catalyzing the oxidation of colorless 3,3,5,5-tetramethylbenzidine (TMB) to generate blue oxidized TMB (oxTMB). Under the presence of AChE and acetylthiocholine chloride (ATCh), the POD-like activity of Pd-Pt MXene was significantly inhibited. The activity of this nanoenzyme could be restored after the addition of AChE inhibitors. Using donepezil as an example, colorimetric detection was conducted within a linear range of 0.1 nmol/L to 10 nmol/L and the lowest detection boundary was only 0.35 nmol/L (S/N = 3). Finally, a paper-based platform was designed and constructed, and it has been successfully employed for AChE inhibitor detection in real samples with the aid of a smartphone. In all, this work paves a new way for designing nanoenzyme-based devices towards medicine determination or screening like AChE inhibitor.