色谱法
萃取(化学)
分馏
降水
蛋白质纯化
产量(工程)
体积热力学
样品制备
化学
悬挂(拓扑)
杂质
蛋白质沉淀
材料科学
数学
有机化学
物理
冶金
气象学
纯数学
量子力学
同伦
作者
Petr Novák,Vladimı́r Havlı́ček
出处
期刊:Elsevier eBooks
[Elsevier]
日期:2016-01-01
卷期号:: 51-62
被引量:112
标识
DOI:10.1016/b978-0-444-63688-1.00004-5
摘要
Efficient extraction of proteins is a key factor for successful proteomic experiments. Samples used for proteomic investigations may be in form of protein solution, e.g. body fluids, cell suspension such as cell cultures or solid samples such as tissues. Each type of sample, i.e. hydrophobic v. hydrophilic, will require different approach to achieve the best yield of extraction. Final volume of extracted proteins will depend on technique used and amount of initial material. In many instances sample volume needs to be reduced for which various techniques are available. Simple concentration in vacuum will cause that all impurities and salts will be concurrently concentrated and may interfere with further steps of sample fractionation. Therefore, protein precipitation techniques are discussed.
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