诱导多能干细胞
生物
胚胎干细胞
科斯尔
细胞生物学
干细胞
胚状体
定向微分
细胞分化
内胚层
肝细胞
生物化学
体外
基因
作者
Philip Roelandt,Jolien Vanhove,Catherine M. Verfaillie
出处
期刊:Methods in molecular biology
日期:2013-01-01
卷期号:: 141-147
被引量:22
标识
DOI:10.1007/978-1-62703-348-0_11
摘要
Differentiation of human stem cells to hepatocytes is crucial for industrial applications as well as to develop new therapeutic strategies for liver disease. The protocol described here, using sequentially growth factors known to play a role in liver embryonic development, efficiently differentiates human embryonic stem cells (hESC) as well as human-induced pluripotent stem cells (hiPSC) to hepatocytes by directing them through defined embryonic intermediates, namely, mesendoderm/definitive endoderm and hepatoblast and hepatocyte phenotype. After 28 days, the final differentiated progeny is a mixture of cells, comprising cells with characteristics of hepatoblasts and a smaller cell fraction with morphological and phenotypical features of mature hepatocytes. An extensive functional characterization of the stem cell progeny should be used to confirm that differentiated cells display functional characteristics of mature hepatocytes including albumin secretion, glycogen storage, and several detoxifying functions such as urea production, bilirubin conjugation, glutathione S-transferase activity, cytochrome activity and drug transporter activity.
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