低温保存
男科
生物
后代
乳糖
突变体
绿色荧光蛋白
分子生物学
生物化学
胚胎
基因
遗传学
医学
怀孕
作者
Ena Nakatsukasa,Naomi Kashiwazaki,Akiko Takizawa,Masao Shino,Kazuhiro Kitada,Tadao Serikawa,Yoji Hakamata,Eiji Kobayashi,Riichi Takahashi,Masatsugu Ueda,Tetsuo Nakashima,Naomi Nakagata
出处
期刊:PubMed
日期:2003-12-01
卷期号:53 (6): 639-41
被引量:13
摘要
We attempted to cryopreserve spermatozoa from closed colonies (Jcl:SD and Jcl:Wistar), and inbred (BN/Crj, F3441 DuCrj, LEW/Crj, Long-Evans and WKY/NCrj), mutant (Zitter [WTC.ZI-zi] and Tremor [TRM]), transgenic (human A-transferase [A], and green fluorescent protein [GFP]) strains of rats. Rat epididymal spermatozoa suspended in cryopreservation solution (23% egg yolk, 8% lactose monohydrate, and 0.7% Equex Stm, pH 7.4, adjusted with 10% Tris [hydroxymethy] aminomethane) were frozen and stored at -196 degrees C. After thawing at 37 degrees C, the spermatozoa were instilled into the tip of each uterine horn of the recipients. A total of five recipient females for each strain were inseminated with cryopreserved spermatozoa, and normal live offspring of all strains (Jcl:SD: 11, Jcl:Wistar: 13, BN/Crj: 9, F344/DuCrj: 28, LEW/Crj: 4, Long-Evans: 6, WKY/NCrj: 8, TRM: 24, WTC.ZI-zi: 27, A: 30 and GFP: 20) were obtained.
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