佐剂
实验性自身免疫性脑脊髓炎
脑脊髓炎
免疫系统
免疫学
生物
弗氏佐剂
抗原
髓鞘碱性蛋白
髓鞘少突胶质细胞糖蛋白
髓鞘
抗体
免疫疗法
医学
体内
作者
Carol Chase Huizar,Niannian Ji,Robert Reddick,Gary R. Ostroff,Thomas G. Forsthuber
标识
DOI:10.1016/j.cellimm.2021.104383
摘要
Abstract For over 70 years experimental autoimmune encephalomyelitis (EAE) has been induced with myelin autoantigens emulsified in complete Freund’s adjuvant (CFA) which has significant side effects such as pain, inflammation, and tissue necrosis at the injection site. β-1,3- d -glucan particles (GPs) are hollow microcapsules prepared from Saccharomyces cerevisiae cell walls that induce potent Th17 cell responses without causing strong injection site tissue reactions. We evaluated the potential of GPs complexed with neuroantigens to induce EAE while avoiding undesirable side effects. GPs loaded with myelin oligodendrocyte glycoprotein 35–55 (MOG35–55) or proteolipid protein 139–151 (PLP139–151) peptides effectively induced EAE in C57BL/6 mice and SJL mice. Disease severity, CNS pathology and immune responses were comparable between GP- and CFA-immunized mice. Importantly, injection with GPs resulted in significantly decreased inflammation compared with CFA. We posit that use of GPs provides an alternative means for inducing EAE that results in comparable disease, but less discomfort to animals.
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