Enhanced Cryopreservation Efficacies of Ice Recrystallization Inhibiting Nanogels

Development of new cryopreservation technologies holds significant potential to revolutionize the fields of cell culture, tissue engineering, assisted reproduction, and transfusion medicine. The current gold standard small-cell permeating cryopreservation agents (CPAs) demonstrate promising cryopreservation efficacies but are cytotoxic and immunogenic at the concentrations required for cryopreservation applications. In comparison, new cell impermeable CPAs of nanodimensions demonstrate outstanding potential to overcome the drawbacks of existing CPAs. In this study, we report the synthesis of vitamin B5 analogous methacrylamide (B5AMA)-incorporated nanogels as a potential solution to address the commonly observed limitations of existing CPAs. The stimuli-responsive poly(B5AMA) nanogels prepared by radical polymerization demonstrated significant ice recrystallization inhibition efficacies and showed either superior or comparable cryopreservation efficacies compared to the traditional cryoprotectant DMSO/glycerol in both eukaryotic and prokaryotic cells.