细胞凋亡
氧化应激
血红素
转染
内科学
内分泌学
分子生物学
谷胱甘肽
生物
神经元
免疫学
血红素
医学
生物化学
酶
基因
神经科学
作者
Yi Liu,Changhong Tan,Weina Li,Xi Liu,Xin Wang,Yuejiang Gui,Qin Lu,Fen Deng,HU Chang-lin,Lifen Chen
出处
期刊:Neuroreport
[Lippincott Williams & Wilkins]
日期:2020-08-13
卷期号:31 (15): 1065-1071
被引量:5
标识
DOI:10.1097/wnr.0000000000001510
摘要
Background A growing body of experimental evidence suggests that hemin released from heme is a potent oxidant and accumulates in intracranial hematomas. Hemopexin (Hpx) decreases hemin accumulation and catabolism by nerve cells. In previous study, we observed that Hpx gene knockout aggravated striatal injury and worsened behavioral deficits of mice subjected to intracerebral hemorrhage. Aim To examine the effect of Hpx on oxidative damage and apoptosis in cultured nerve cells with blood clot. Methods Neuron and glial cells were transfected with adenoviral Hpx gene. Transfected primary neuron-glial cells were co-cultured with 50 μl of arterial blood clot using insert transwells. The sham group was co-coulture with 50 μl of DMEM/F12, which contained 28 μl of serum; the control group was transfected with adenoviral vector. At 12 and 24 h, the level of malonaldehyde (MDA), surperoxide dismutase (SOD) concentration, glutathione (GSH), apoptosis, expression of HO-1 and caspase-3 were detected. Results MDA level was decreased ( P < 0.01) whereas SOD and GSH concentration were increased in the Hpx group ( P < 0.05 and P < 0.01, respectively). Results of flow cytometry revealed no significant difference in apoptosis between the Hpx group and model group at 12 h. However, the percentage of cells undergoing apoptosis in the Hpx group was decreased at 24 h compared with the model group ( P < 0.01). HO-1 expression decreased in the Hpx group at 24 h ( P < 0.01) while caspase-3 expression decreased at both 12 and 24 h ( P < 0.011 and P < 0.05, respectively) compared with the model group. Conclusion Hpx protected nerve cells exposed to blood from injury by anti-oxidation and a decrease in the expression of HO-1 and caspase-3.
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