劈理(地质)
二硫键
化学
键裂
立体化学
生物化学
生物
催化作用
断裂(地质)
古生物学
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:1965-12-17
卷期号:150 (3703): 1595-1598
被引量:129
标识
DOI:10.1126/science.150.3703.1595
摘要
Disulfide bonds can be cleaved at an alkaline p H by treating a protein with excess of a reagent disulfide in the presence of catalytic amounts of thiol. The cleavage products are stable and can be isolated; they contain the mixed disulfide between the reagent and the exposed thiol groups of the protein. The extent of cleavage is readily controlled by the p H of the reaction, temperature, and the addition of urea. Disulfide bonds cleaved by the reaction can be re-formed by exposing the mixed disulfide of the protein to catalytic amounts of thiol. Specific side chains can be added on to the thiol groups in native proteins by treatment with a reagent disulfide alone.
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