甲烷菌
甲烷八叠球菌
古细菌
厌氧消化
拟杆菌
生物
微生物种群生物学
细菌
食品科学
环境化学
化学
甲烷
生态学
16S核糖体RNA
遗传学
作者
Yafan Cai,Bin Hua,Lijuan Gao,Yuncai Hu,Xufeng Yuan,Zongjun Cui,Wanbin Zhu,Xiaofen Wang
标识
DOI:10.1016/j.biortech.2017.04.071
摘要
Although trace elements are known to aid anaerobic digestion, their mechanism of action is still unclear. High-throughput sequencing was used to reveal the mechanism by which adding trace elements affects microbial communities and their action. The results showed that the highest methane yields, with addition of Fe, Mo, Se and Mn were 289.2, 289.6, 285.3, 293.0 mL/g volatile solids (VS), respectively. The addition of Fe, Mo, Se and Mn significantly (P < 0.05) reduced the level of volatile fatty acids (VFAs). The dominant bacteria and archaea were Bacteroidetes and Methanosaeta, respectively. Compared with the proportion of Methanosaeta in the control group, treatment with added trace elements increased Methanosaeta by as much as 12.4%. Microbial community analysis indicated that adding trace elements changed the composition and diversity of archaea and bacteria. Methane yield was positively correlated with bacterial diversity and negatively correlated with archaeal diversity for most treatments.
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