叶绿体
基因组工程
质体
碳纳米管
生物
转基因
基因传递
拟南芥
烟草
植物
生物物理学
细胞生物学
基因组
基因
纳米技术
Cas9
材料科学
生物化学
突变体
遗传增强
作者
Seon‐Yeong Kwak,Tedrick Thomas Salim Lew,Connor J. Sweeney,Volodymyr B. Koman,Min Hao Wong,Karen Bohmert-Tatarev,Kristi D. Snell,Jun Sung Seo,Nam‐Hai Chua,Michael S. Strano
标识
DOI:10.1038/s41565-019-0375-4
摘要
Plant genetic engineering is an important tool used in current efforts in crop improvement, pharmaceutical product biosynthesis and sustainable agriculture. However, conventional genetic engineering techniques target the nuclear genome, prompting concerns about the proliferation of foreign genes to weedy relatives. Chloroplast transformation does not have this limitation, since the plastid genome is maternally inherited in most plants, motivating the need for organelle-specific and selective nanocarriers. Here, we rationally designed chitosan-complexed single-walled carbon nanotubes, utilizing the lipid exchange envelope penetration mechanism. The single-walled carbon nanotubes selectively deliver plasmid DNA to chloroplasts of different plant species without external biolistic or chemical aid. We demonstrate chloroplast-targeted transgene delivery and transient expression in mature Eruca sativa, Nasturtium officinale, Nicotiana tabacum and Spinacia oleracea plants and in isolated Arabidopsis thaliana mesophyll protoplasts. This nanoparticle-mediated chloroplast transgene delivery tool provides practical advantages over current delivery techniques as a potential transformation method for mature plants to benefit plant bioengineering and biological studies.
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