磷酰胺
寡核苷酸合成
寡核苷酸
DNA合成
计算生物学
计算机科学
合成生物学
纳米技术
生物
DNA
遗传学
材料科学
作者
Lifu Song,Zhenghua Deng,Zi-Yi Gong,Lulu Li,Bingzhi Li
标识
DOI:10.3389/fbioe.2021.689797
摘要
Over the past decades, remarkable progress on phosphoramidite chemistry-based large-scale de novo oligonucleotide synthesis has been achieved, enabling numerous novel and exciting applications. Among them, de novo genome synthesis and DNA data storage are striking. However, to make these two applications more practical, the synthesis length, speed, cost, and throughput require vast improvements, which is a challenge to be met by the phosphoramidite chemistry. Harnessing the power of enzymes, the recently emerged enzymatic methods provide a competitive route to overcome this challenge. In this review, we first summarize the status of large-scale oligonucleotide synthesis technologies including the basic methodology and large-scale synthesis approaches, with special focus on the emerging enzymatic methods. Afterward, we discuss the opportunities and challenges of large-scale oligonucleotide synthesis on de novo genome synthesis and DNA data storage respectively.
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