黄萎病
生物
大丽花黄萎病
数量性状位点
黄萎病
基因座(遗传学)
棉属
候选基因
近交系
遗传学
基因
植物
作者
Yuxiang Wang,Jing Zhao,Qin Chen,Kai Zheng,Xiaojuan Deng,Wenju Gao,Wenfeng Pei,Shiwei Geng,Yahui Deng,Chunping Li,Quanjia Chen,Yanying Qu
出处
期刊:Plant Science
[Elsevier]
日期:2023-02-01
卷期号:327: 111562-111562
被引量:4
标识
DOI:10.1016/j.plantsci.2022.111562
摘要
Improving resistance to Verticillium wilt is of great significance for achieving high and stable yields of Upland cotton (Gossypium hirsutum). To deeply understand the genetic basis of cotton resistance to Verticillium wilt, Verticillium wilt-resistant Upland Lumianyan 28 and four Verticillium wilt-susceptible Acala cotton cultivars were used to create four recombinant inbred line (RIL) populations of 469 families through nested hybridization. Phenotypic data collected in five stressful environments were used to select resistant and sensitive lines and create a mixed pool of extreme phenotypes for BSA-seq. A total of 8 QTLs associated with Verticillium wilt resistance were identified on 4 chromosomes, of which qVW-A12-5 was detected simultaneously in the RIL populations and in one of the RIL populations and was identified for the first time. According to the sequence comparison and transcriptome analysis of candidate genes in the QTL interval between parents and pools, 4 genes were identified in the qVW-A12-5 interval. qRT-PCR of parental and phenotypically extreme lines revealed that Gh_CPR30 was induced by and may be a candidate gene for resistance to Verticillium wilt in G. hirsutum. Furthermore, VIGS technology revealed that the disease severity index (DSI) of the Gh_CPR30-silenced plants was significantly higher than that of the control. These results indicate that the Gh_CPR30 gene plays an important role in the resistance of G. hirsutum to Verticillium wilt, and the study provides a molecular basis for analyzing the molecular mechanism underlying G. hirsutum resistance to Verticillium wilt.
科研通智能强力驱动
Strongly Powered by AbleSci AI