纳米传感器
化学
谷胱甘肽
生物物理学
细胞毒性
线粒体
氧化还原
赫拉
纳米颗粒
罗丹明
纳米技术
费斯特共振能量转移
活性氧
生物化学
细胞
荧光
体外
无机化学
物理
酶
生物
材料科学
量子力学
作者
Ying Lian,Rui Jiang,Zeyu Zhang,Zhenzhen Lin,Nianhong Wang,Xudong Wang
标识
DOI:10.1021/acs.analchem.2c00855
摘要
Mitochondrial glutathione (mGSH) is both the cause of the oxidative damage and a mechanism for maintaining the redox homeostasis in mitochondria. To effectively measure mGSH dynamics in living cells, we have developed a new FRET-based nanosensor by immobilizing rhodamine B into dendritic mesoporous silica nanoparticles and installing GSH probes and mitochondria-targeting motifs onto the surface of nanoparticles. The result shows that these nanosensors show efficient FRET and a full reversibility and rapid response (<10 s) to GSH in the range of 0.5–20 mM, due to their unique nanostructure and well-overlapped spectra. The excellent photostability and low cytotoxicity make them an effective means for monitoring mGSH concentration in real time. When the mGSH nanosensors are used for quantitatively measuring mGSH variations under glucose deprivation stimulation in HeLa cells, they successfully prove themselves a useful tool for mitochondrial redox activity studies.
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