医学
离体
体内
胶质瘤
荧光寿命成像显微镜
核医学
前瞻性队列研究
胶质母细胞瘤
放射科
临床前影像学
脑瘤
队列
病理
荧光
癌症研究
生物技术
物理
生物
量子力学
作者
Deling Li,Jingjing Zhang,Chongwei Chi,Xiong Xiao,Junmei Wang,Lixin Lang,Imran Ali,Gang Niu,Liwei Zhang,Jie Tian,Nan Ji,Zhaohui Zhu,Xiaohong Chen
出处
期刊:Theranostics
[Ivyspring International Publisher]
日期:2018-01-01
卷期号:8 (9): 2508-2520
被引量:124
摘要
Purpose: Despite the use of fluorescence-guided surgery (FGS), maximum safe resection of glioblastoma multiforme (GBM) remains a major challenge.It has restricted surgeons between preoperative diagnosis and intraoperative treatment.Currently, an integrated approach combining preoperative assessment with intraoperative guidance would be a significant step in this direction.Experimental design: We developed a novel 68 Ga-IRDye800CW-BBN PET/near-infrared fluorescence (NIRF) dual-modality imaging probe targeting gastrin-releasing peptide receptor (GRPR) in GBM.The preclinical in vivo tumor imaging and FGS were first evaluated using an orthotopic U87MG glioma xenograft model.Subsequently, the first-in-human prospective cohort study (NCT 02910804) of GBM patients were conducted with preoperative PET assessment and intraoperative FGS.Results: The orthotopic tumors in mice could be precisely resected using the near-infrared intraoperative system.Translational cohort research in 14 GBM patients demonstrated an excellent correlation between preoperative positive PET uptake and intraoperative NIRF signal.The tumor fluorescence signals were significantly higher than those from adjacent brain tissue in vivo and ex vivo (p < 0.0001).Compared with pathology, the sensitivity and specificity of fluorescence using 42 loci of fluorescence-guided sampling were 93.9% (95% CI 79.8%-99.3%)and 100% (95% CI 66.4%-100%), respectively.The tracer was safe and the extent of resection was satisfactory without newly developed neurologic deficits.Progression-free survival (PFS) at 6 months was 80% and two newly diagnosed patients achieved long PFS.Conclusions: This initial study has demonstrated that the novel dual-modality imaging technique is feasible for integrated pre-and intraoperative targeted imaging via the same molecular receptor and improved intraoperative GBM visualization and maximum safe resection.
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