谷胱甘肽
谷胱甘肽还原酶
高氯酸
化学
酶
生物化学
色谱法
酵母
谷胱甘肽过氧化物酶
有机化学
作者
Douglas O. Adams,Chandrika Liyanage
标识
DOI:10.5344/ajev.1991.42.2.137
摘要
In the course of adapting an enzymatic method for the spectrophotometric determination of glutathione in grapevines, we found that certain tissues, especially the rachis, contained a powerful inhibitor of yeast glutathione reductase (EC 1.6.4.2) used for the assay. Further experimentation showed that the inhibitor could be removed from 5% perchloric acid extracts by adding polyvinylpolypyrrolidone (PVPP) after adjusting the pH of the extract to 3.5 with 2 N KOH. This modification allows the assay of glutathione in leaves, rachis, and berries. Recovery of glutathione from perchloric acid extracts treated with PVPP was shown to be nearly quantitative and values for glutathione in Thompson Seedless berries obtained using the assay were in the range previously reported for other varieties.
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