线粒体
内化
癌细胞
细胞凋亡
细胞毒性
化学
细胞
细胞生物学
纳米技术
配体(生物化学)
生物物理学
体外
癌症
材料科学
生物化学
生物
受体
遗传学
作者
Jiangsheng Xu,Fang Zeng,Hao Wu,Caiping Hu,Changmin Yu,Shuizhu Wu
出处
期刊:Small
[Wiley]
日期:2014-05-15
卷期号:10 (18): 3750-3760
被引量:114
标识
DOI:10.1002/smll.201400437
摘要
The therapeutic applications of exogenous nitric oxide are usually limited by its short half‐life and its vulnerability to many biological substances, thus straightforward and precise spatiotemporal control of NO delivery may be critical to its therapeutic effects. Herein, the mitochondria‐targeted and photoresponsive NO‐releasing nanosystem is demonstrated as a new approach for cancer treatment. The nanosystem is fabricated by covalently incorporating a NO photo‐donor and a mitochondria targeting ligand onto carbon‐dots; accordingly, multi‐functionalities (mitochondria‐targeting, light‐enhanced efficient NO‐releasing, and cell imaging) are achieved. The in vitro NO release profiles for the nanosystem show that the duration of NO release from the present C‐dot‐based nanosystem containing immobilized SNO can be extended up to 8 hours or more. Upon cellular internalization, the nanosystem can target mitochondria and release NO. The action of the nanosystem on three cancer cell lines is evaluated; it is found that the targeted NO‐releasing system can cause high cytotoxicity towards the cancer cells by specifically damaging their mitochondria. Additionally, light irradiation can amplify the cell apoptosis by enhancing NO release. These observations demonstrate that incorporating mitochondria‐targeting ligand onto a NO‐releasing system can enhance its pro‐apoptosis action, thereby providing new insights for exploiting NO in cancer therapy.
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