生物
小虾
活性氧
基因敲除
过氧化氢酶
先天免疫系统
微生物学
平衡
肠道菌群
小肠
RNA干扰
肠粘膜
免疫系统
细胞生物学
免疫学
抗氧化剂
生物化学
内科学
生态学
核糖核酸
细胞凋亡
基因
医学
作者
Huiting Yang,Ming-Chong Yang,Jiejie Sun,Fang Guo,Jiang-Feng Lan,Xianwei Wang,Xiao‐Fan Zhao,Jinxing Wang
标识
DOI:10.1016/j.fsi.2015.08.021
摘要
Intestinal innate immune response is an important defense mechanism of animals and humans against external pathogens. The mechanism of microbiota homeostasis in host intestines has been well studied in mammals and Drosophila. The reactive oxygen species (ROS) and antimicrobial peptides have been reported to play important roles in homeostasis. However, how to maintain the microbiota homeostasis in crustacean intestine needs to be elucidated. In this study, we identified a novel catalase (MjCAT) involved in ROS elimination in kuruma shrimp, Marsupenaeus japonicus. MjCAT mRNA was widely distributed in hemocytes, heart, hepatopancreas, gills, stomach, and intestine. After the shrimp were challenged with pathogenic bacteria via oral infection, the expression level of MjCAT was upregulated, and the enzyme activity was increased in the intestine. ROS level was also increased in the intestine at early time after oral infection and recovered rapidly. When MjCAT was knocked down by RNA interference (RNAi), high ROS level maintained longer time, and the number of bacteria number was declined in the shrimp intestinal lumen than those in the control group, but the survival rate of the MjCAT-RNAi shrimp was declined. Further study demonstrated that the intestinal villi protruded from epithelial lining of the intestinal wall were damaged by the high ROS level in MjCAT-knockdown shrimp. These results suggested that MjCAT participated in the intestinal host-microbe homeostasis by regulating ROS level.
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