下调和上调
糖尿病性心肌病
内科学
内分泌学
2型糖尿病
糖尿病
链脲佐菌素
免疫印迹
心肌病
基因表达
胰岛素
基因
医学
心肌细胞
基因敲除
心力衰竭
生物
遗传学
作者
Dayi Qin,Biao Huang,Lili Deng,Hala El-Adawi,Kalyan Ganguly,James R. Sowers,Nabil El‐Sherif
标识
DOI:10.1006/bbrc.2001.4825
摘要
Type I diabetic cardiomyopathy has consistently been shown to be associated with decrease of repolarising K(+) currents, but the mechanisms responsible for the decrease are not well defined. We investigated the streptozotocin (STZ) rat model of type I diabetes. We utilized RNase protection assay and Western blot analysis to investigate the message expression and protein density of key cardiac K(+) channel genes in the diabetic rat left ventricular (LV) myocytes. Our results show that message and protein density of Kv2.1, Kv4.2, and Kv4.3 are significantly decreased as early as 14 days following induction of type I diabetes in the rat. The results demonstrate, for the first time, that insulin-deficient type I diabetes is associated with early downregulation of the expression of key cardiac K(+) channel genes that could account for the depression of cardiac K(+) currents, I(to-f) and I(to-s). These represent the main electrophysiological abnormality in diabetic cardiomyopathy and is known to enhance the arrhythmogenecity of the diabetic heart. The findings also extend the extensive list of gene expression regulation by insulin.
科研通智能强力驱动
Strongly Powered by AbleSci AI