材料科学
角膜
间质细胞
3D生物打印
生物医学工程
组织工程
眼科
医学
癌症研究
作者
Gyeong Won Lee,Ajeesh Chandrasekharan,Subhadeep Roy,Akash Thamarappalli,Binapani Mahaling,H G Lee,Keum‐Yong Seong,Sourabh Ghosh,Seung Yun Yang
出处
期刊:Biofabrication
[IOP Publishing]
日期:2024-03-20
标识
DOI:10.1088/1758-5090/ad35eb
摘要
Abstract 3D bioprinting has the potential for the rapid and precise engineering of hydrogel constructs that can mimic the structural and optical complexity of a healthy cornea. However, the use of existing light-activated bioinks for corneal printing is limited by their poor cytocompatibility, use of cytotoxic photoinitiators, low photo-crosslinking efficiency, and opaque/colored surface of the printed material. Herein, we report a fast-curable, non-cytotoxic, optically transparent bioprinting system using a new water-soluble benzoyl phosphinate-based photoinitiator and photocrosslinkable methacrylated hyaluronic acid (HAMA). Compared with commercially available photoinitiators, the newly developed photoinitiator, lithium benzoyl(phenyl) phosphinate (BP), demonstrated increased photoinitiation efficiency under visible light and low cytotoxicity. Using a catalytic amount of BP, the HA-based bioinks quickly formed 3D hydrogel constructs under low-energy visible-light irradiation (405 nm, < 1 J/cm2). The mechanical properties and printability of photocurable bioinks were further improved by blending low (10 kDa) and high (100 kDa) molecular weight (MW) HAMA by forming multilength networks. For potential applications as corneal scaffolds, stromal cell-laden dome-shaped constructs were fabricated using MW-blended HAMA/BP (bHAMA/BP) bioink and a digital light processing printer. The HA-based photocurable bioinks exhibited good cytocompatibility (80~95%), fast curing kinetics (<5 s), and excellent optical transparency (>90% in the visible range), potentially making them suitable for corneal tissue engineering.
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