RNA序列
核糖核酸
生物
巨病毒
进化生物学
基因
基因组
遗传学
基因表达
转录组
作者
Amir Fromm,Gur Hevroni,Flora Vincent,Daniella Schatz,Carolina A. Martínez-Gutiérrez,Frank Aylward,Assaf Vardi
出处
期刊:Nature microbiology
日期:2024-04-11
标识
DOI:10.1038/s41564-024-01669-y
摘要
Giant viruses (phylum Nucleocytoviricota) are globally distributed in aquatic ecosystems. They play fundamental roles as evolutionary drivers of eukaryotic plankton and regulators of global biogeochemical cycles. However, we lack knowledge about their native hosts, hindering our understanding of their life cycle and ecological importance. In the present study, we applied a single-cell RNA sequencing (scRNA-seq) approach to samples collected during an induced algal bloom, which enabled pairing active giant viruses with their native protist hosts. We detected hundreds of single cells from multiple host lineages infected by diverse giant viruses. These host cells included members of the algal groups Chrysophycae and Prymnesiophycae, as well as heterotrophic flagellates in the class Katablepharidaceae. Katablepharids were infected with a rare Imitervirales-07 giant virus lineage expressing a large repertoire of cell-fate regulation genes. Analysis of the temporal dynamics of these host–virus interactions revealed an important role for the Imitervirales-07 in controlling the population size of the host Katablepharid population. Our results demonstrate that scRNA-seq can be used to identify previously undescribed host–virus interactions and study their ecological importance and impact. Active infections of giant viruses in their marine protists hosts are tracked at single-cell resolution, showing that, despite being rare, these viruses still impact microbial population dynamics.
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