作者
Shin Ae Lee,Jun Heo,Mi Ae Kim,Tomohiko Tamura,Satomi Saitou,Soon-Wo Kwon,Hang-Yeon Weon
摘要
Two bacterial strains, FWR-8 T and CFWR-9 T , were isolated from the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea. Both strains were strictly aerobic, Gram-stain-positive and non-motile. Strain FWR-8 T possessed the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13 T and the phylogenetic tree revealed that strain FWR-8 T formed a cluster with Ptb. intestinalis 2DFWR-13 T . Pseudolysinimonas kribbensis MSL-13 T and Lysinimonas yzui N7XX-4 T shared a high 16S rRNA gene sequence similarity (97.8 %) and formed a cluster adjacent to the cluster that included Ptb. intestinalis 2DFWR-13 T . The 16S rRNA gene sequence of strain CFWR-9 T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353 T and the phylogenetic tree indicated that strain CFWR-9 T formed one independent cluster with A. binzhouensis OAct353 T that was within the radius of the genus Agromyces . The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8 T were characterized as type B1, iso-C 16 : 0 , anteiso-C 15 : 0 and anteiso-C 17 : 0 , MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively. Those from strain CFWR-9 T were type B1, iso-C 16 : 0 , anteiso-C 15 : 0 and anteiso-C 17 : 0 , MK-11, MK-12 and MK-10, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively. Based on the phenotypic and genotypic data, strains FWR-8 T and CFWR-9 T each represent a novel species within the genera Protaetiibacter and Agromyces , respectively. For these species, the names Protaetiibacter larvae sp. nov. and Agromyces intestinalis sp. nov. have been proposed, with the type strains FWR-8 T (=KACC 19322 T =NBRC 113051 T ) and CFWR-9 T (=KACC 19306 T =NBRC 113046 T ), respectively. Our results also justify a reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb. nov. and an emended description of the genus Pseudolysinimonas isprovided.