角质形成细胞
细胞凋亡
流式细胞术
细胞生物学
生物
细胞质
半胱氨酸蛋白酶
线粒体
程序性细胞死亡
分子生物学
细胞培养
生物化学
遗传学
作者
Yoram Soroka,Ze’evi Ma’or,Yael Leshem,Lilian Verochovsky,Rami Neuman,François Brégégère,Yoram Milner
标识
DOI:10.1016/j.exger.2008.08.003
摘要
The aging process and its characterization in keratinocytes have not been studied in depth until now. We have assessed the cellular and molecular characteristics of aged epidermal keratinocytes in monolayer cultures and in skin by measuring their morphological, fluorometric and biochemical properties. Light and electron microscopy, as well as flow cytometry, revealed increase in cell size, changes in cell shape, alterations in mitochondrial structure and cytoplasmic content with aging. We showed that the expression of 16 biochemical markers was altered in aged cultured cells and in tissues, including caspases 1 and 3 and β-galactosidase activities, immunoreactivities of p16, Ki67, 20S proteasome and effectors of the Fas-dependent apoptotic pathway. Aged cells diversity, and individual variability of aging markers, call for a multifunctional assessment of the aging phenomenon, and of its modulation by drugs. As a test case, we have measured the effects of Dead Sea minerals on keratinocyte cultures and human skin, and found that they stimulate proliferation and mitochondrial activity, decrease the expression of some aging markers, and limit apoptotic damage after UVB irradiation.
科研通智能强力驱动
Strongly Powered by AbleSci AI