Detection of H2O2 and redox potential of an acute wound of rabbits with different sugar intakes based on a SERS-active microneedle

To detect H2O2 and redox potential of an acute wound simultaneously, a SERS-active microneedle was fabricated by integrating H2O2 and redox potential SERS probes into two grooves of an acupuncture needle, respectively. When the SERS-active microneedle was inserted into tissues, an acute wound was formed and the two SERS probes were introduced into the wound to sense H2O2 and redox potential of the wound. The feasibility of the SERS-active microneedle was evaluated ex vivo and in vivo. Both sugar deficiency and sugar excess induce increases in H2O2 and redox potential in most tissues. Short-term physiological disturbances eventually returned to normal levels in healthy organisms, and abnormal sugar intakes disrupt the normal physiological environment and induce stress responses in all tissues. The SERS-active microneedle for detection of H2O2 and redox potential would become a powerful tool, used for collecting physiological signals in different tissues to elucidate the mechanisms of wound healing and redox-related diseases.