化学
异牡荆素
色谱法
透析
血浆蛋白结合
血液蛋白质类
类黄酮
生物化学
内科学
牡荆素
医学
抗氧化剂
作者
Nan Ding,Chao Chen,Yishu Liu,Peiyong Zheng,Xue Li,Ming Yang
标识
DOI:10.1016/j.ab.2024.115511
摘要
The total flavonoids of Desmodium styracifolium (TFDS) are flavonoid-rich extracts obtained from Desmodii Styracifolii Herba, which is approved for the treatment of urolithiasis in China. C-glycosylflavones including schaftoside, vicenin-1, vicenin-2, vicenin-3, and isovitexin are the main active constituents. In this study, the plasma protein binding of these compounds was determined for the first time in rat and human plasma by rapid equilibrium dialysis combined with HPLC-MS/MS method. The developed method was validated in terms of specificity, linearity, accuracy, precision, extraction effect, matrix effect, and stability. Schaftoside, vicenin-1, vicenin-2, and vicenin-3 exhibited moderate plasma protein binding, ranging from 56.6% to 61.5% in rat plasma and 55.0%–62.9% in human plasma. In comparison, isovitexin demonstrated a higher plasma protein binding in the range of 92.3–93.1% and 95.1–96.2% in rat and human plasma, respectively. Furthermore, the potential interactions mediated via plasma protein binding between isovitexin and nonsteroidal anti-inflammatory drugs (NSAIDs) were investigated by rapid equilibrium dialysis. No significant changes were observed, indicating a lower likelihood of interaction between TFDS and NSAIDs due to plasma protein binding in the treatment of urinary system disorders.
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