汤剂
类风湿性关节炎
传统医学
医学
药理学
超滤(肾)
内科学
化学
色谱法
作者
Weiliang Fu,Chengyu Shentu,Dan Chen,Junjie Qiu,Chuhong Zong,Hengyuan Yu,Yiwei Zhang,Yong Chen,Xuesong Liu,Tengfei Xu
标识
DOI:10.1016/j.jep.2024.118268
摘要
Shaoyao Gancao Fuzi Decoction (SGFD), has been employed for thousands of years in the treatment of rheumatoid arthritis (RA) with remarkable clinical efficacy. However, the material basis underlying the effectiveness of SGFD still remains unclear.This study aims to elucidate the material basis of SGFD through the application of network pharmacology and biological affinity ultrafiltration.UPLC-Q-TOF-MS/MS was employed to characterize the components in SGFD, the identified 145 chemical components were mainly categorized into alkaloids, flavonoids, triterpenoids, and monoterpenoids according to the structures. Network pharmacology method was utilized to identify potential targets and signaling pathways of SGFD in the RA treatment, and the anti-inflammatory and anti-RA effects of SGFD were validated through in vivo and in vitro experiments. Moreover, as the significant node in the pharmacology network, TNF-α, a classical therapeutic target in RA, was subsequent employed to screen the interacting compounds in SGFD via affinity ultrafiltration screening method, 6 active molecules (i.e.,glycyrrhizic acid, paeoniflorin, formononetin, isoliquiritigenin, benzoyl mesaconitine, and glycyrrhetinic acid) were exhibited significant interactions. Finally, the significant anti-inflammatory and anti-TNF-α effects of these compounds were validated at the cellular level.In conclusion, this study comprehensively elucidates the pharmacodynamic material basis of SGFD, offering a practical reference model for the systematic investigation of traditional Chinese medicine formulas.
科研通智能强力驱动
Strongly Powered by AbleSci AI