Currently, 2D MXenes are recognized as potential materials because of their miscellaneous chemical composition with excellent physical, electronic, mechanical, magnetic, and thermal properties. The MXenes family has attracted significant research interest in various application fields, for instance, agriculture, environmental remediation, energy storage, and biomedical, due to their excellent features, such as hydrophilicity, activated metallic hydroxide sites, high surface area, biocompatibility, and ease of functionalization. The investigation of biomedical applications is comparatively more escalating than in other fields. Hence, in this book chapter, the synthesis methods, application, characterization techniques, and properties of 2D MXenes are presented, along with the challenges and future opportunities of MXenes.