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The mechanism of damage to the posterior silk gland by trace amounts of acetamiprid in the silkworm, Bombyx mori

啶虫脒 家蚕 丝素 丝绸 化学 新烟碱 毒性 分子生物学 生物 杀虫剂 毒理 基因 生物化学 益达胺 生态学 材料科学 复合材料 有机化学
作者
Zhengting Lu,Mengxue Li,Yingying Fang,Jianwei Qu,Wentao Ye,Minli Dai,Dandan Bian,Tingting Mao,Fanchi Li,Hongjuan Sun,Bing Li
出处
期刊:Pesticide Biochemistry and Physiology [Elsevier BV]
卷期号:170: 104676-104676 被引量:11
标识
DOI:10.1016/j.pestbp.2020.104676
摘要

Acetamiprid is a new neonicotinoid insecticide widely used in the prevention and control of pests in agriculture. However, its residues in the environment affect the cocooning of the silkworm, Bombyx mori (B. mori), a non-target insect. To investigate the mechanism of damage, B. mori larvae were fed with trace amounts of acetamiprid (0.15 mg/L). At 96 h after exposure, the larvae showed signs of poisoning and decreased body weight, resulting in reduced survival and ratio of cocoon shell. At 48 h and 96 h after exposure, the residues in the posterior silk gland (PSG), which is responsible for synthesizing silk fibroin, were 0.72 μg/mg and 1.21 μg/mg, respectively, as measured by high performance liquid chromatography, indicating that acetamiprid can accumulate in the PSG. Moreover, pathological sections and transmission electron microscopy also demonstrate the damage of the PSG by acetamiprid. Digital gene expression (DGE) and KEGG pathway enrichment analysis revealed that genes related to metabolism, stress responses and inflammation were significantly up-regulated after exposure. Quantitative RT-PCR analysis showed that the transcript levels of FMBP-1 and FTZ-F1 (transcription factors for synthesizing silk protein) were up-regulated by 2.55-and 1.56-fold, respectively, and the transcript levels of fibroin heavy chain (Fib-H), fibroin light chain (Fib-L), P25, Bmsage and Bmdimm were down-regulated by 0.75-, 0.76-, 0.65-, 0.44- and 0.40-fold, respectively. The results indicate that accumulated acetamiprid causes damage to the PSG and leads to reduced expression of genes responsible for synthesizing silk fibroin. Our data provide reference for evaluating the safety of acetamiprid residues in the environment for non-target insects.
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