明胶
基质凝胶
球体
自愈水凝胶
3D生物打印
组织工程
癌细胞
生物医学工程
生物物理学
材料科学
化学
细胞
体外
纳米技术
癌症
细胞生物学
生物
生物化学
医学
高分子化学
遗传学
作者
Salvador Flores-Torres,Omar Peza-Chavez,Hellen Kuasne,Jose G. Munguia-Lopez,Jacqueline Kort-Mascort,Lorenzo Ferri,Tao Jiang,Charles V. Rajadurai,Morag Park,Veena Sangwan,Joseph M. Kinsella
出处
期刊:Biofabrication
[IOP Publishing]
日期:2021-03-10
卷期号:13 (2): 025001-025001
被引量:33
标识
DOI:10.1088/1758-5090/abdb87
摘要
Hydrogels consisting of controlled fractions of alginate, gelatin, and Matrigel enable the development of patient-derived bioprinted tissue models that support cancer spheroid growth and expansion. These engineered models can be dissociated to be then reintroduced to new hydrogel solutions and subsequently reprinted to generate multigenerational models. The process of harvesting cells from 3D bioprinted models is possible by chelating the ions that crosslink alginate, causing the gel to weaken. Inclusion of the gelatin and Matrigel fractions to the hydrogel increases the bioactivity by providing cell-matrix binding sites and promoting cross-talk between cancer cells and their microenvironment. Here we show that immortalized triple-negative breast cancer cells (MDA-MB-231) and patient-derived gastric adenocarcinoma cells can be reprinted for at least three 21 d culture cycles following bioprinting in the alginate/gelatin/Matrigel hydrogels. Our drug testing results suggest that our 3D bioprinted model can also be used to recapitulatein vivopatient drug response. Furthermore, our results show that iterative bioprinting techniques coupled with alginate biomaterials can be used to maintain and expand patient-derived cancer spheroid cultures for extended periods without compromising cell viability, altering division rates, or disrupting cancer spheroid formation.
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