内体
液泡
ESCRT公司
细胞生物学
液泡蛋白分选
内吞作用
排序nexin
TSG101型
绿色荧光蛋白
转运蛋白
突变体
化学
蛋白质靶向
自噬
生物
生物化学
膜蛋白
膜
细胞质
基因
微泡
细胞
细胞凋亡
小RNA
细胞内
作者
Mingjing Luo,Ying Zhu,Zhiqi Liu,Liwen Jiang
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 15-21
标识
DOI:10.1007/978-1-0716-0767-1_2
摘要
Endocytosis and endosomal trafficking to vacuoles play important roles in regulating the homeostasis of plasma membrane (PM) proteins in plant cells. FREE1 (FYVE domain protein required for endosomal sorting 1) is a plant-unique component of the ESCRT (endosomal sorting complex required for transport) machinery. In free1 mutant plants, PIN-FORMED 2 (PIN2)-GFP was found to mislocalize from the PM to the tonoplast. In this chapter, we describe a detailed protocol for studying vacuolar sorting and degradation of PIN2-GFP by using T-DNA insertional mutants, dexamethasone (DEX) inducible RNAi lines, and other tools, including Fei-Mao (FM) dye staining and dark treatment. By using these methods, we illustrate the endosomal trafficking and vacuolar degradation of PIN2-GFP in plants.
科研通智能强力驱动
Strongly Powered by AbleSci AI