类有机物
神经毒性
细胞生物学
银纳米粒子
化学
神经突
神经干细胞
诱导多能干细胞
细胞生长
肌球蛋白
细胞
细胞凋亡
干细胞
生物
胚胎干细胞
生物化学
纳米技术
纳米颗粒
毒性
体外
材料科学
有机化学
基因
作者
Yan Huang,Lulu Guo,Chulin Cao,Rui Ma,Yu-Ting Huang,Kai Zhong,Hong Gao,Yina Huang,Qian Bu
标识
DOI:10.1016/j.scitotenv.2022.157047
摘要
Silver nanoparticles (AgNPs) are used in various research fields. Although the neurotoxicity of AgNPs has been explored in animal models and 2D cell-culture models, including human stem cells, these models cannot accurately mimic the development of the human brain. Therefore, the potential mechanisms of AgNPs-induced developmental neurotoxicity in humans are still largely unclear. In this study, cerebral organoids derived from induced pluripotent stem cells were treated with 0.1 μg/mL or 0.5 μg/mL AgNPs for 7 days. At the low concentration (0.1 μg/mL), AgNPs increased the cell proliferation and inhibited the neural apoptosis in the organoids, but impaired the cilium assembly and elongation, which may perturb the cell cycle and induce abnormal cerebral-organoid growth. Conversely, at the high concentration (0.5 μg/mL), AgNPs significantly inhibited cell proliferation and induced apoptosis in cerebral organoids. High-concentration AgNPs reduced the expression and co-localization of the cytoskeleton proteins F-actin, myosin, and tubulin, thereby perturbing neurite growth. In conclusion, AgNPs exposure induces developmental neurotoxic effects in cerebral organoids and is thus a potential congenital risk factor.
科研通智能强力驱动
Strongly Powered by AbleSci AI